Lentil lectin-unrelated reactivity of Aleuria aurantia lectin in affinity electrophoresis of alpha-fetoprotein.
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چکیده
منابع مشابه
Lectin affinity electrophoresis of alpha-fetoprotein detected by immunoenzymatic and chemiluminescent amplification followed by direct scanning.
Microdetermination of alpha-fetoprotein (AFP) glycoforms by lectin affinity electrophoresis followed by chemiluminescence reaction using horseradish peroxidase (POD) or alkaline phosphatase (ALP) in antibody-affinity blotting was developed. The intensity of chemiluminescence obtained by ALP was greater than that by POD; however, the coefficient of variation with POD was less than that with ALP....
متن کاملLentil-lectin-reactive alpha-fetoprotein in the differential diagnosis of benign and malignant liver disease.
Affinity chromatography of serum on lentil lectin bound to Sepharose 4B has been used to identify different forms of alpha-fetoprotein in serum of patients with liver disease. We studied eight patients with non-malignant liver disease, finding that 7-15% of the serum alpha-fetoprotein bound to the lectin. In contrast, for 15 patients with malignant liver disease, 25-83% of the serum alpha-fetop...
متن کاملInfluence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL
Protein-carbohydrate interactions are very often mediated by the stacking CH-π interactions involving the side chains of aromatic amino acids such as tryptophan (Trp), tyrosine (Tyr) or phenylalanine (Phe). Especially suitable for stacking is the Trp residue. Analysis of the PDB database shows Trp stacking for 265 carbohydrate or carbohydrate like ligands in 5 208 Trp containing motives. An app...
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We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using...
متن کاملFractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin.
The carbohydrate-binding specificity of Aleuria aurantia lectin was investigated by analyzing the behavior of a variety of fucose-containing oligosaccharides on an A. aurantia lectin-Sepharose column. Studies with complex-type oligosaccharides obtained from various glycoproteins by hydrazinolysis and their partial degradation fragments indicated that the presence of the alpha-fucosyl residue li...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1991
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.35.265